CRISPR technologies paving the way for advances in regenerative medicine https://www.byteseu.com/825509/ #Cas9 #Cell #CRISPR #CysticFibrosis #Fibrosis #GENE #Genes #Genetic #Medicine #OsteogenesisImperfecta #ProgenitorCells #Research #SickleCellDisease #StemCells #Technology
SciTech Chronicles. . . . . . . . .Feb 15th, 2025
#Diabetes #antibiotic #resistance #infection ##Murchison #airplane #reflection #RFI CRISPR-Cas9 #Cas9 #RNA #metagenomics #Dragonfly #Titan #Methane #Ingenuity #Londinium #tribunal #70AD #preservation
Researchers at the Broad Institute of MIT and Harvard and Cyrus Biotechnology have now #engineered two #CRISPR nucleases, #Cas9 and #Cas12, to mask them from the #immune system.
#Biotechnology #Biomedical #sflorg
https://www.sflorg.com/2025/01/biot01092501.html
après l'achat de toutes les maisons d'édition de France au bénéfice d'une famille de miliardaires, le mégaéditeur acquert aussi le labo du MIT qui fait de l'édition génétique.
Neue biotechnologische Methoden erlauben punktgenaue Eingriffe in das Erbgut – wie gut lassen sich Veränderungen nachweisen?
Der aktuelle Wissensstand im #BfR2GO: https://www.bfr.bund.de/epaper/bfr2go_02_2023_deutsch/#page=22
#Bacteriophages encode anti-CRISPR proteins that inactivate bacterial #CRISPR-Cas systems, allowing successful invasion, replication & integration. This study shows that #antiCRISPR protein AcrIIA1 induces degradation of #Cas9 in human cells #PLOSBiology https://plos.io/3uUGAos
Scientists at Oak Ridge National Laboratory used their expertise in #quantum #biology, artificial intelligence and #bioengineering to improve how #CRISPR #Cas9 #genome editing tools work on #organisms like #microbes that can be modified to produce #renewable #fuels and #chemicals.
#Biology #AI #ArtificialIntelligence #sflorg
https://www.sflorg.com/2023/11/bio11072301.html
Find out what happens when Merkulov et al. combine #Cas9-targeted #Nanopore sequencing (CANS) with the novel pipeline NanoCasTE to trace both #genetically inherited and #somatic #TEIs in plants!
https://doi.org/10.1111/jipb.13555
@wileyplantsci
#JIPB #PlantSci #Arabidopsis #PlantScience #botany
They call it “#skeletal #editing” and they hope it will change their field – and our world.
Inspired in part by the revolutionary genome-editing technology #Crispr-#Cas9, Levin and Sarpong are among a handful of chemists developing new methods to #insert, #delete and #swap individual atoms within molecules.
The word “editing” evokes chemists altering atoms with pairs of nanoscopic tweezers, but that would be far from efficient. “If you wanted to make a mole,” Levin explains, referring to a unit of measurement used in chemistry, “you would have to take that pair of tweezers and do it 10^23 times.”
Compact Cas9d and HEARO enzymes for genome editing discovered from uncultivated microbes
#Polarity of the #CRISPR roadblock to #transcription | Nature Structural & Molecular Biology
Improved bacterial single-cell RNA-seq through automated MATQ-seq and Cas9-based removal of rRNA reads | bioRxiv
Target site preferences mean that no single #Cas9 can edit all sequences. Study identifies PI domain variants in natural SaCas9 orthologs that recognize a simple NNGG PAM; these can confer NNGG PAM recognition, expanding the #CRISPR repertoire #PLOSBiology https://plos.io/3gJB4OR
Target site preferences mean that no single #Cas9 can edit all sequences. Study identifies PI domain variants in natural SaCas9 orthologs that recognize a simple NNGG PAM; these can confer NNGG PAM recognition, expanding the #CRISPR repertoire #PLOSBiology https://plos.io/3gJB4OR
Target site preferences mean that no single #Cas9 can edit all sequences. Study identifies PI domain variants in natural SaCas9 orthologs that recognize a simple NNGG PAM; these can confer NNGG PAM recognition, expanding the #CRISPR repertoire #PLOSBiology https://plos.io/3gJB4OR
Our new #bacterial #scRNAseq preprint is out, building on the Vogel lab's MATQ-seq approach. We automate to increase cell numbers, increase sensitivity, and deplete rRNA using #Cas9, before applying this to gene expression heterogeneity along the #Salmonella growth curve.
Aktualisierte Fragen und Antworten zu Genome Editing und CRISPR/Cas9: https://www.bfr.bund.de/de/fragen_und_antworten_zu_genome_editing_und_crispr_cas9-199684.html
J'ai regardé un chouette #documentaire hier soir sur ces fameux "ciseaux génétiques" dont on a entendu parler récemment suite au prix #Nobel de chimie décerné aux chercheuses Emmanuelle #Charpentier et Jennifer #Doudna .
C'était une bonne occasion d'en apprendre un peu plus et de comprendre en quoi ça consistait.
Je l'ai trouvé passionnant et très bien réalisé ce petit documentaire. 
https://mega.nz/file/CfI3UQTS#bLbCUBR1bx3xdNQsO-ZUWHklof3hEAKtuzk_XUkCrU0
High prevalence of Streptococcus pyogenes #Cas9-reactive T cells within the adult human population | #immunity #CRISPRCas9 #crispr https://www.nature.com/articles/s41591-018-0204-6
